摘要
通过中和试验证明 ,B34腹水单克隆抗体中和 IBDV CA株的中和效价高达 1∶42 0 0 0 0。经 1 0倍稀释后 ,能等量中和含毒量为 1 0 6.62 5TCID50 /ml的 IBDV CA株细胞毒。利用 B34腹水单克隆抗体建立了 MAb- AC- ELISA,可用于测定 IBDV CA株细胞毒的病毒含量 ,与 TCID50之间的相关系数为 0 .
Through neutralization test,B34 MAb neutralized IBDV CA strain,with the titre of neutralization antibody in B34 ascite so high as 1∶420000. When diluted 1∶10 and mixed with equal volume of virus ,B34 ascite can completely neutralize the IBDV CA strain containing virus at least 105.625 TDIC 50 /0.1ml.A MAb based antigen capture ELISA (AC ELISA) was developed to titrate IBDV CA strain and the correlation coefficient between AC ELISA titre and TDIC 50 was 0.94.
出处
《中国兽药杂志》
北大核心
2000年第2期13-15,共3页
Chinese Journal of Veterinary Drug
