期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Gata4转录后蛋白共价修饰 被引量：5

Post-transcriptional protein modification of Gata4
原文传递
导出
摘要 Gata4是心脏发育重要的转录因子,其转录活性和DNA亲和力受转录后蛋白共价修饰的调节,并影响下游目的基因和相关转录因子表达,胚胎干细胞分化,心肌细胞生长和心脏发育。该文总结Gata4转录后蛋白共价修饰对其转录活性的影响,寻找其与心脏发育和先天性心脏病(congenital heart disease,CHD)的关系。结果发现乙酰化、磷酸化、SUMO化使其转录活性和DNA亲和力升高,下游基因表达上升,促进胚胎干细胞分化等呈正性调节;去乙酰化和甲基化下调Gata4转录活性,呈负性调节。因此,Gata4蛋白共价修饰对研究先天性心脏病及其他一些心脏疾病有重要的临床意义。 Gata4 is an important transcription factor in heart development.Gata4 post-transcriptional protein modification regulates transcriptional activity and DNA binding,which in turn affects expression of downstream genes and transcription factors,differentiation of embryonic stem cells and cardiogenesis.This article summarizes the effect of post-transcriptional protein modification on transcriptional activity of Gata4 and the relationship between this effect and congenital heart disease.It was shown that acetylation,phosphorylation and SUMOylation upregulate transcriptional activity,DNA binding,downstream gene expression and embryonic stem cell differentiation.On the other hand,methylation and deacetylation downregulate Gata4 transcriptional activity.Post-transcriptional protein modification of Gata4 is very important in clinical research on congenital and other heart diseases.
作者 俞立玮 桂永浩
机构地区 复旦大学附属儿科医院
出处 《中国当代儿科杂志》 CAS CSCD 北大核心 2012年第10期800-802,F0003,共4页 Chinese Journal of Contemporary Pediatrics
基金 国家自然科学基金面上项目 批准号:81170147
关键词 蛋白共价修饰 心脏发生 先天性心脏病 Protein modification Cardiogenesis Congenital heart disease
分类号 R725.4 [医药卫生—儿科]
  • 相关文献

参考文献27

  • 1陈名武,刘唐威,庞玉生.转录因子GATA-4在心血管系统中作用的研究进展[J].中华心血管病杂志,2008,36(1):85-87. 被引量：11
  • 2Molkentin JD.The zinc finger-containing transcription factors GA-TA-4,-5,and-6.Ubiquitously expressed regulators of tissue-spe-cific gene expression[J].J Biol Chem,2000,275(50):38949-38952.
  • 3Pu WT,Ishiwata T,Juraszek AL,Ma Q,Izumo S.GATA4 is adosage-sensitive regulator of cardiac morphogenesis[J].Dev Biol,2004,275(1):235-244.
  • 4Takaya T,Kawamura T,Morimoto T,Ono K,Kita T,ShimatsuA,et al.Identification of p300-targeted acetylated residues in GA-TA4 during hypertrophic responses in cardiac myocytes[J].J BiolChem,2008,283(15):9828-9835.
  • 5Kawamura T,Ono K,Morimoto T,Wada H,Hirai M,Hidaka K,et al.Acetylation of GATA-4 is involved in the differentiation ofembryonic stem cells into cardiac myocytes[J].J Biol Chem,2005,280(20):19682-19688.
  • 6Kaichi S,Takaya T,Morimoto T,Sunagawa Y,Kawamura T,Ono K,et al.Cyclin-dependent kinase 9 forms a complex withGATA4 and is involved in the differentiation of mouse ES cells intocardiomyocytes[J].J Cell Physiol,2011,226(1):248-254.
  • 7Sun H,Yang X,Zhu J,Lv T,Chen Y,Chen G,et al.Inhibitionof p300-HAT results in a reduced histone acetylation and down-regulation of gene expression in cardiac myocytes[J].Life Sci,2010,87(23-26):707-714.
  • 8Trivedi CM,Zhu W,Wang Q,Jia C,Kee HJ,Li L,et al.Hopxand Hdac2 interact to modulate Gata4 acetylation and embryoniccardiac myocyte proliferation[J].Dev Cell,2010,19(3):450-459.
  • 9Li T,Liu Z,Hu X,Ma K,Zhou C.Involvement of ERK-RSKcascade in phenylephrine-induced phosphorylation of GATA4[J].Biochim Biophys Acta,2011,1823(2):582-592.
  • 10Hu X,Li T,Zhang C,Liu Y,Xu M,Wang W,et al.GATA4regulates ANF expression synergistically with Sp1 in a cardiac hy-pertrophy model[J].J Cell Mol Med,2011,15(9):1865-1877.

二级参考文献25

  • 1Huang WY, Heng HH, Liew CC. Assignment of the human GATA4 gene to 8p23. 1→p22 using fluorescence in situ hybridization analysis. Cytogenet Cell Genet, 1996,72:217-218.
  • 2Molkentin JD. The zinc finger-containing transcription factors GATA-4,-5, and-6 ubiquitously expressed regulators of tissuespecific gene expression. J Biol Chem,2000,275:38949-38952.
  • 3Pu WT, Ishiwata T, Juraszek AL, et al. GATA4 is a dosagesensitive regulator of cardiac morphogenesis. Dev Biol, 2004,275: 235 -244.
  • 4Xin M, Davis CA, Molkentin JD, et al. A threshold of GATA4 and GATA6 expression is required for cardiovascular development. Proc Nail Acad Sci U S A, 2006,103:11189-11194.
  • 5Rajagopal SK, Ma Q, Obler D, et al. Spectrum of heart disease associated with murine and human GATA4 mutation. J Mol Cell Cardio1,2007, [ Epub ahead of print].
  • 6Peterkin T, Gibson A, Loose M,et al. The roles of GATA-4, -5 and -6 in vertebrate heart development. Semin Cell Dev Biol, 2005,16:83-94.
  • 7Liang Q, De Windt LJ, Witt SA, et al. The transcription factors GATA4 and GATA6 regulate cardiomyocyte hypertrophy in vitro and in vivo. J Biol Chem, 2001,276:30245-30253.
  • 8Ueyama T, Kawashima S, Sakoda T, et al. Requirement of activation of the extraceUularsignal-regulated kinase cascade in myocardial cell hypertrophy. J Mol Cell Cardiol, 2000,32 : 947- 960.
  • 9Yanazume T, Hasegawa K, Wada H, et al. Rho/ROCK pathway contributes to the activation of extracellular signal-regulated kinase/GATA-4 during myocardial cell hypertrophy. J Biol Chem, 2002,277:8618-8625.
  • 10Tenhunen O, Sarman B, Kerkda R, et al. Mitogen-activated protein kinases p38 and ERK 1/2 mediate the wall stress-induced activation of GATA-4 binding in adult heart. J Biol Chem, 2004, 279 : 24852-24860.

共引文献10

同被引文献76

  • 1Hoffman JIE, Kaplan S. The incidence of congenital heart disease[J]. J Am Coil Cardiol, 2002, 39(12): 1890-1900.
  • 2Pfaff SL, Mendelsohn M, Stewart CL, Edlund T, Jessell TM. Requirement for LIM homeobox gene Isll in motor neuron generation reveals a motor neuron-dependent step in intemeuron differentiation[J]. Cell, 1996, 84(2): 309-320.
  • 3Du A, Hunter CS, Murray J, Noble D, Cai CL, Evans SM, et al. Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas[J]. Diabetes, 2009, 58(9): 2059-2069.
  • 4Laugwitz KL, Moretti A, Caron L, Nakano A, Chien KR. Isletl cardiovascular progenitors: a single source for heart lineages?[J]. Development, 2008, 135(2): 193-205.
  • 5Stevens KN, Hakonarson H, Kim CE, Doevendans PA, Koeleman BP, Mital S, et al. Common variation in ISL1 confers genetic susceptibility for human congenital heart disease[J]. PLoS One, 2010, 5(5): e10855.
  • 6Langwitz KL, Moretti A, Lain J, Gruber P, Chen Y, Woodard S, et al. Insulin gene enhancer binding protein Isl-1 is a member ofa novel class of proteins containing both a hom, eo-and a Cys His domain[J]. Nature, 1990, 344(6269): 879-882.
  • 7Barat-Houari M, Clement K, Vatin V, Dina C, Bonhomme G, Vasseur F, etal. Positional candidate gene analysis of Lim domain homeobox gene (Isl-1) on chromosome 5ql 1-q13 in a French morbidly obese population suggests indication for association with type 2 diabetes[J]. Diabetes, 2002, 51 (5): 1640-1643.
  • 8Barzelay A, Ben-Shoshan J, Entin-Meer M, Maysel-Auslender S, Afek A, Barshack I, et al. A potential role for islet-1 in post- natal angiogenesis and vasculogenesis[J]. Thromb Haemost, 2010, 103(1): 188-197.
  • 9Moretti A, Caron L, Nakano A, Lam Jr, Bemshausen A, Chen Y, et al. Multipotent embryonic isll+ progenitor cells lead to cardiac, smooth muscle, and endothelial cell diversification[J]. Cell, 2006, 127(6): 1151-1165.
  • 10Cai CL, Liang X, Shi Y, Chu PH, Pfaff SL, Chen J, et al. Isll identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of ceils to the heart[J]. Dev Cell, 2003, 5(6): 877-889.

引证文献5

二级引证文献13

中国当代儿科杂志
2012年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部