康复训练对血管性痴呆大鼠海马β淀粉样多肽及胰岛素降解酶的影响

he effects of rehabilitation training on amyloid-beta peptide and insulin-degrading enzyme levels in the hip pocampus of rats with vascular dementia

目的观察康复训练对血管性痴呆（VD）大鼠海马B一淀粉样多肽（Aβ）及胰岛素降解酶（IDE）的影响。方法共选取30只SD大鼠，采用随机数字表法将其分为康复组、模型组及假手术组。选用结扎双侧颈总动脉方法制成VD大鼠模型，康复组每天进行1h康复训练。于术后第4周进行行为学测试，以评估各组大鼠学习记忆能力；待行为学测试结束后采用免疫组化法检测各组大鼠海马（DG）区Aβ及IDE表达。结果术后第4周时发现康复组大鼠学习记忆功能明显优于模型组（P〈0．05）；且康复组大鼠海马区Aβ表达较模型组显著降低（P〈0．05），IDE表达则较模型组明显增高（P〈0．05）。结论康复训练能改善YD大鼠学习记忆功能，其治疗机制可能与上调IDE表达、减少Aβ在海马中的蓄积有关。

Objective To investigate the effects of rehabilitation training on hippocampal amyloid-beta pep- tide （Aβ） and insulin-degrading enzyme （IDE） levels in vascular dementia （VD）. Methods Thirty female Spra- gue-Dawley rats were randomly assigned to a rehabilitation group （n = 10） , a model group （n = 10） or a sham-opera- tion group （n = 10）. An experimental VD model was established in the rats of the first 2 groups by bilateral common carotid artery permanent ligation. The rats in the rehabilitation group then received i h of rehabilitation training daily. Learning and memory were assessed at 4 weeks after the operation. Immunohistoehemical staining was used to detect Aβ and IDE expression in the hippocampus dentate gyrus （DG） area. Results The rats in the rehabilitation group showed significantly better learning ability compared with the model group. The expression of Aβ in the rehabilitation group was significantly less than in the model group. The expression of IDE in the rehabilitation group was significant- ly greater. Conclusion Rehabilitation can accelerate the recovery of learning and memory in VD, at least in rats. The mechanism is possibly related to decreased accumulation of Aβ in the hippocampus due to up-regulation of the expression of IDE.