摘要
目的对免疫透射比浊法定量测定降钙素原的方法进行实验评价。方法实验评价包括精密度、线性、回收实验及干扰实验。结果该方法测定降钙素原线性良好(y=1.152x-0.0452,r=0.999),批内CV<5%,批间CV<10%;回收率平均97.7%;对胆红素、血红蛋白及三酰甘油具有较好的干扰能力。结论该方法简便、快速,便于自动化检测,适合临床使用。
Objective To evaluate the method of procalcitonin detection by immunoturbidmetric assay.Methods Immunoturbidmetric assay was established to determine serum procalcitonin,and then its precision,linearity,recovery and interference test were evaluated.Results The method had a good linearity response（y=1.152x-0.0452）,and the linear correlation coefficient r=0.999.The inter and intra CVs of the serum procalcitonin levels were lower than 5% and 10%,respectively.The average recovery was 97.7%.Some degree of bilirubin,hemoglobin and triglycerides did not interfere with the procalcitonin results significantly.Conclusion Immunoturbidmetric assay is a simple,fast and automated detection method,which shows good performance in clinical use.
出处
《安徽医学》
2012年第9期1222-1223,共2页
Anhui Medical Journal
关键词
降钙素原
免疫透射比浊法
方法学评价
精密度
线性
干扰
Procalcitonin
Immunoturbidmetric assay
Methodological evalution
Precision
Linearity
Interference
