结肠癌中MMP-11和Moesin mRNA的表达与肿瘤转移及预后的关系

Expression of mRNA of matrix metalloproteinase 11 and Moesin and its relevance to metastasis and prognosis in colorectal carcinoma

目的检测结肠癌中基质金属蛋白酶-11(matrix metalloproteinase 11,MMP-11)和膜突蛋白(Moesin)mRNA表达水平并探讨其表达与肿瘤转移和预后的关系。方法采用原位杂交方法检测68例结肠癌组织及40例正常结肠组织中MMP-11和Moesin mRNA的表达情况。结果结肠癌组织中MMP-11和Moesin mRNA表达显著高于正常组织(P<0.05);癌组织中MMP-11和Moesin mRNA表达与组织学分化、淋巴结转移和临床分期有关,MMP-11表达还与浸润深度相关;MMP-11 mRNA阳性表达患者5年生存率(46.8%)明显低于阴性表达患者(75%),差异有统计学意义(P<0.05),但Moesin mRNA阳性表达患者五年生存率与阴性表达患者差异无统计学意义(P>0.05)。结论 MMP-11和Moesin mRNA高表达与结肠癌的浸润和转移密切相关,其中MMP-11可以作为结肠癌预后的指标。

Objective To determine the expression of mRNA of matrix metalloproteinase 11 （ MMP-11 ） and Moesin in colorectal carcinoma tissue, and to investigate the correlation between its expression and tumor metastasis and prognosis. Methods The expressions of mRNA of MMP-11 and Moesin in 68 tissues from patients with colorectal carcinoma and 40 tissues with normal colorectal mucosa were detected with in situ hybridization. Results The positive expression of mRNA of MMP-11 and Moesin in colorectal carcinoma tissue was 69.1%, and 60.3 % respectively, which were both significantly higher than that in normal colorectal mucosa （P 〈 0.05）. The expression of mRNA of MMP-11 and Moesin was correlated with histologic differentiation, lymph node metastasis and clinical staging in patients with colorectal carcinoma. The expression of MMP-11 mRNA was also correlated with the depth of invasion of colorectal carcinoma into tissues （P 〈0.05）. The 5-year survival rates were 46.8% and 75% in colorectal carcinoma patients with positive and negative expression of MMP-11 mRNA, respectively. The difference was statistically significant （P 〈 0.05）. There were no significant differences on the 5-year survival rates between the colorectal carcinoma patients with positive and negative expression of Moesin mRNA. Conclusion The expressions of mRNA of MMP-11 and Moesin are correlated with the progression and metastasis of colorectal carcinoma, and MMP-11 may be used as a predictor for prognosis of colorectal carcinoma.