摘要
目的对结核病临床诊断方法的应用价值进行比较分析。方法对别采用涂片法、免疫磁珠技术分离涂片法(IMS-涂片)、痰培养法、荧光定量聚合酶链反应(FQ-PCR)对病例组112例和对照组53例痰标本进行检测。结果普通涂片法灵敏度最低为25.00%,其次是IMS-涂片和痰培养分别是39.29%和37.50%,FQ-PCR法的灵敏度为67.86%,明显高于其他方法。结论 FQ-PCR法和IMS-涂片可作为普通抗酸染色法和培养法的重要补充或是互补的方法,对提高检出率,早发现、早治疗极具临床意义。
Objective To perform the comparative analysis on the application value of the clinical diagnostic methods of tuberculosis. Methods The smear, immune magnetic beads technology separation smear(IMS-smear), sputum culture and FQ PCR meth~,ds were adopted to detect the sputum specimens in 112 cases of tuberculosis and 53 control cases. Results The sensitivity of the common smear method was lowest(25.00%), which of the IMS- smear and the sputum cuhurc were 39. 29% and 37. 50% respectively. The sensitivity of FQ-PCR was 67. 86%, which was significantly higher than that of other mt.thods. Conclusion FQ-PCR and iMS-smear can be used as an important supplement of common acid fast staining and euhure methods or complementary approach, which has great clinical significance to increase the detection rate,early discover and early treat tuberculosis.
出处
《检验医学与临床》
CAS
2012年第20期2577-2578,共2页
Laboratory Medicine and Clinic
关键词
结核分枝杆菌
结核
抗酸染色
免疫磁珠技术
结核培养
荧光定量聚合酶链反应
Mycobacterium tuberculosis
tuberculosis
acid fasl staining
immunomagnetic beads tech-nique
tuberculosis culture
FQ-PCR