摘要
本文合成了荧光探针香草醛缩苯胺,该探针的荧光可被羟基自由基(·OH)猝灭,藕合Fenton反应,建立了一种荧光猝灭法测定羟基自由基的新方法。该方法的检测线性范围为2.00×10-6-2.00×10-5mol·L-1,检出限为8.00×10-7mol·L-1。利用该方法测定了几种大豆提取液对羟基自由基清除作用,结果满意。
A new fluorescent probe reagent N - [ 3 - methoxy - 4 - hydroxyphenylmenthylidene ] - aniline was synthesized. Combination of Fenton reaction, a new fluorescence quenching method for determination of hy- droxyl radical （ · OH） was proposed. The linear range and detection limit are 2.00.×10^-6-2.00×10^-5mol·L^-1 and 8.00 × 10^ -7. The spectrofluorometric method was applied to determine the scavenging effect of several soy- beans. The results were satisfactory.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2012年第4期517-521,共5页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
羟基自由基(·OH)
香草醛缩苯胺
荧光猝灭
Hydroxyl radical （ · OH）
N - [ 3 - methoxy - 4 - hydroxyphenylmenthylidene ] - aniline
Fluores-cence quenching
