日粮类型对猪肌肉组织HSL mRNA和Obese mRNA基因表达的影响

Effects of Diet Type on Expression of HSL mRNA and Obese mRNA in Muscle Tissue of Pigs

选用20 kg左右的"杜×长×大"外三元杂交仔猪20头,随机分为2组,分别饲喂豆粕型和杂粕(双低菜籽粕+豆粕)型2种不同类型的日粮,饲养猪至体重达到100 kg左右时屠宰测定瘦肉率,取背最长肌肌肉组织,采用RT-PCR技术检测分析HSL mRNA和Obese mRNA表达情况,以探索出日粮类型与基因表达的关系。结果显示,杂粕日粮较豆粕日粮可显著增加HSL mRNA表达量、减少Obese mRNA表达量(P<0.05),2种日粮类型虽对猪瘦肉率无显著性影响,但是从实际值来看,杂粕日粮表现出提高趋势,较豆粕日粮提高3.5%(P>0.05)。

Twenty Duroc x Landrace x large three crossbred pigs were allocated randomLy into two groups and fed soybean diet and miscellane- ous diet （ double low rapeseed meal and soybean meal）. The pigs weight were reached about one hundred kilogram when slaughter test slaughtered and determined lean lean rate, in addition, taked the longissimus dorsi muscle tissue, The levels of HSL mRNA and Obese mRNA were detected by RT - PCR to explore relationship of diet type and gene expression. The results showed that miscellaneous meal with soybean meal in the diets significantly in- creased HSL expression of mRNA, decreased Obese expression of mRNA （ miscellaneous meal with soybean meal in the diets significantly increased HSL expression of mRNA, decreased Obese expression of mRNA （ P 〈 0.05 ） , Between soybean diet and miscellaneous diet on pig lean meat rate has no significant impact, but the actual data showed that pig fed miscellaneous diet showed a tendency to increase 3.5% （ P 〉 0.05 ） , comparisoning of pig fed soybean diet.