柱穗山羊草α-醇溶蛋白基因的克隆与序列分析

Cloning and Sequence Analysis of α-Gliadin Genes from Aegilops cylindrica

根据已知的普通小麦α-醇溶蛋白基因序列设计引物,采用PCR方法克隆基因并进行序列分析。从柱穗山羊草Y127中克隆得到1个α-醇溶蛋白基因序列Gli2-Z-2,它具有α-醇溶蛋白基因的典型结构特征,编码区全长939 bp,编码313个氨基酸。氨基酸序列比较显示,Gli2-Z-2在多聚谷氨酰胺区比已报道的α-醇溶蛋白序列有较多的谷氨酰胺残基。

The specific PCR primers were designed based on the known wheat α-gliadin gene se- quences, and were used to amplify the coding genes of α-gliadin from Aegilops cylindrica. The coding regions of Gli2 - Z - 2 were isolated from Aegilops cylindrica Y127. The full coding region of Gli2 - Z - 2 was 939 bp, and could be translated into a protein of 313 amino acids. The cloned gliadin genes had the typical structure of α-gliadin genes. The deduced amino acid sequences comparison suggested that Gli2 -Z -2 had more Gluta- mine than the known α-gliadin genes.