蜡梅枝叶化学成分及其抗病毒活性

Chemical Constituents from the Branches and Leaves of Chimonanthus praecox with Antiviral Activity Investigations

利用各种色谱技术从蜡梅枝叶中共分离得到10个化合物,通过波谱学方法将其鉴定为(+)-Calycan-thine(1)、(-)-Folicanthine(2)、(-)-Chimonanthine(3)、异秦皮啶(4)、3,3'-双异秦皮啶(5)、Euoniside(6)、山奈酚-3-O-芸香糖苷(7)、3,4-二羟基苯乙腈(8)、Di-O-methylcrenatin(9)和Acanthoside B(10)。化合物4～10为首次从该植物中发现。采用细胞病变效应法测试了化合物1～5与8对猪繁殖与呼吸综合征病毒的抑制活性,化合物2(IC50=58.9±10.2μM;TI=19.3)、3(IC50=68.9±3.1μM;TI=17.9)与8(IC50=80.5±16.9μM;TI>19.9)显示出了弱的抑制活性。

Ten compounds were isolated from the branches and leaves of Chimonanthus praecox by various chromato- graphic techniques. By spectroscopic methods ,their structures were elucidated as （ ＋ ）-calyeanthine （1）, （-）-foliean- thine （2）, （-） -chimonanthine （ 3 ）, isofraxidin （ 4 ）, 3,3＇-biisofraxidin （ 5 ）, euoniside （ 6 ）, kaempferel-3-O- rutinoside （7）,3,4-dihydroxybenzonitrile （8）, di-O-methylcrenatin （9） and acanthoside B （10）. Among them, compounds 4-10 were obtained from the herb for the first time. The inhibitory activity of compounds 1-5 and 8 against porcine respiratory and reproductive syndrome virus （PRRSV） was measured by the cytopathic effect （CPE） method. Compounds 2 （IC50= 58.9±10.2 μM;TI = 19.3）,3 （IC50= 68.9±3.1 μM;TI = 17.9） and8 （IC5o = 80.5±16.9 μM;TI 〉 19.9） showed weak effect on PRRSV.