超高效液相色谱-串联质谱法同时测定饲料中的7种β_2-兴奋剂

Simultaneous Determination of 7 Kinds of β_2-agonist in Feed by UPLC-MS/MS

为了准确测定饲料中7种β2-兴奋剂,建立了超高效液相色谱-串联质谱检测方法。样品经酸性有机混合溶剂提取、固相萃取净化浓缩后,在多反应监测模式下,用带电喷雾离子源(ESI)的串联质谱仪对样品进行定性和定量分析。该方法下各化合物定量检出限为50μg/kg。在50、100、200μg/kg的添加浓度下,回收率在71%～103%之间,相对标准偏差在5.7%～9.8%之间。本方法快速、准确、灵敏度高,适合饲料中福莫特罗、苯乙醇胺A、克仑特罗、莱克多巴胺、沙丁胺醇、特布它林、西马特罗的同时快速检测。

A method of ultra performance liquid chromatograghy-electrospray tandem mass spectrometry （UPLC-MS/MS） was studied for determination of β2-agonist in feed. The sample was extracted by 0.1 mol/L hydrochloric acid-methanol mixed solvent, purified and enriched with cationic solid phase extraction column, and which was separated by liquid chromatography on C18 column with mobile phase of 0.1% formic acid in methanol（gradient elution）, then analyzed in UPLC-MS/MS with electrospray ion source（ESI） under multi-re- action monitor mode （MRM）. The quantification was performed with external standard method. The method has a lower limit of quantitation 50 μg/kg. Spike recoveries of three concentration addition levels（50, 100, 200 μg/kg） ranged in 71%--103% with relative standard deviations in 5.7%--9.8%. The method is fast, accurate and sensitive. It is suit- able for rapid qualitative and quantitative detection of β2-agonist in animal feed.