细胞培养室空气中微生物种类、数量及药物敏感性分析

Species,number,and drug sensitivity of microbes in air samples from a cell culture laboratory

目的研究细胞培养室内空气中微生物种类及药物敏感性,人员操作前、后和采用紫外线照射或甲醛熏蒸消毒后数量变化情况。方法人员操作前、后及紫外线照射或甲醛熏蒸后采用自然沉降法对细胞培养室内空气进行采样,进行细菌培养和菌落计数,并鉴定微生物种类和药物敏感性。结果人员操作前、后和消毒后培养室内采集的空气样品经培养后分别有54、205和42个菌落生长,经鉴定为12属22种细菌和2种真菌,其中革兰阳性菌6属13种,革兰阴性菌6属9种,优势菌依次为藤黄微球菌(M.luteus)、解糖葡萄球菌(S.saccharolyticus)、西地西菌种-3(Cedecea.Spe-cies-3)和表皮葡萄球菌(S.epidermidis)。其中对测试抗生素无耐药性的细菌有4种,分别是藤黄微球菌、解糖葡萄球菌、西地西菌种-3和头状葡萄球菌头状亚种(S.capitis);对1、2和4种(或以上)种类抗生素耐药的细菌分别有4、4和8种。结论人员操作前、后细胞培养室内空气中细菌数量变化极大。细胞培养室内优势细菌对常见抗菌素尚敏感,紫外线照射和甲醛熏蒸均能减少细胞培养室内空气中微生物数量。

Objectives To identify species and antibiotic sensitivity of the microorganisms in air samples from a cell culture laboratory and to determine changes in their number before and after laboratory work by technicians as well as after disinfection with ultraviolet radiation or fumigation with methanaL Methods Microorganisms in the air samples from the laboratory were collected by the natural sedimentation method before and after laboratory work by technicians as well as after disinfection with ultraviolet radiation or fumigation with methanal. They were then cultured, identified, and test- ed against antibiotics. The number of microbe colonies cultured in medium was counted at different times. Results There were 54,205 microbe colonies in the culture medium before and after laboratory work by technicians and 42 after disinfection. There were 22 species of bacteria that included microbes from 12 genera and 2 species of fungi. Among these bacteria species, there were 13 species of Gram-positive bacilli from 6 genera and 9 species of Gram-negative bacilli from 6 genera. The predominant microbes were M. luteus, S. saccharolyticus, Cedecea species 3, and S. epidermidis. The an- tibiotics sensitivity test showed these four bacteria species, i. e. M. luteus, S. saccharolyticus, Cedecea species 3, and S. capitis, were not drug-resistant. Four of the isolated microbes were resistant to 1 antibiotic, 4 were resistant to 2 antibi otics, and 8 were resistant to 4 antibiotics. Conclusion The number of microorganisms in the air samples from the cell culture laboratory varied greatly with work by technicians. The most common microbes are still sensitive to common anti-microbials. The number of microorganisms in the air of a cell culture laboratory can easily be decreased by ultraviolet radiation or fumigation with methanal.