沙棘粗多糖对正常和造模糖尿病小鼠血糖影响的研究

Seabuckthorn Polysaccharide's Effect on Blood Glucose of Normal Mice and Modeling Diabetic Mice

目的探讨沙棘粗多糖对正常小鼠耐糖量的影响,以及对造模糖尿病小鼠高血糖的影响。方法采用小鼠耐糖量模型,以沙棘粗多糖为实验药物,观察其对蔗糖、葡萄糖、麦芽糖、淀粉负荷剂量后的正常小鼠血糖影响;分别用四氧嘧啶、链脲佐菌素诱发实验性糖尿病动物模型,不同剂量的沙棘粗多糖灌胃,观察对高血糖小鼠血糖的影响。结果小鼠耐糖量模型组与对照组比较,淀粉组血糖值降低较为明显(P<0.01),蔗糖组次之(P<0.05);与四氧嘧啶(链脲佐菌素)模型组比较,给药2周和4周后,沙棘粗多糖高剂量组对高血糖小鼠降糖作用明显(P<0.01,P<0.05),降糖率分别为31.72%(33.14%)和45.70%(43.78%),沙棘粗多糖低剂量组降糖作用次之(P<0.05,P<0.05),降糖率分别为28.02%(27.07%)和39.38%(36.43%)。结论沙棘粗多糖能够提高正常小鼠的耐糖量;沙棘粗多糖对四氧嘧啶和链脲佐菌素致高血糖小鼠具有良好的降血糖作用,且作用强度与剂量呈相关性。

modeling Objective The paper aims to study seabuckthorn polysaccharide＇s effect on blood glucose of normal mice and diabetic mice by doing experiment. Methods On one hand, the paper tries to adopt it as a test drug and then tries to find its effects upon blood glucose of mice after they are administered with a loading dose of sucrose, glucose, maltose, and starch. On the other hand, the paper tries to observe its effects on blood sugar of hyperglycemic mice after diabetic mice model, respectively induced by alloxan and streptozotocin, which are administered intra-gastrically with different doses of seabuckthorn polysaccharide. Results We find following results. One is that compared with the control group, in the glucose tolerance model group, the starch group obviously lowers their blood glucose level（P 〈0.01）, and next to it is the sucrose group（P 〈0.05）. The other is that compared with alloxan（streptozotocin）, the high-dozed model group with seabuckthorn polysaccharide produces an obvious effect upon hyperglycemic mice after being administered for two weeks and four weeks（P 〈0.01, P 〈0.05）. And the last result is that their sugar reducing rate is respectively 31.72%（33.14%） and 45.70%（43.78%）, only followed by low-dozed model group（P 〈0.05, P 〈0.05）, with sugar reducing rates respectively as 28.02%（27.07%） and 39.38%（36.43%）. Gonclusion We draw a conclusion that seabuckthoru has a good effect upon reducing blood sugar of mice induced by alloxan and streptozotocin respectively, but hypoglycemic intensity is related to dosage.