刺五加喷干粉改善果蝇睡眠作用的量-效和时-效关系研究

Dose-Effect and Time-Effect Relationship of Ciwujia Spray-Dried Powder on Sleep inprovement of Drososophila

目的观察刺五加喷干粉对果蝇睡眠觉醒周期的影响,探讨刺五加喷干粉改善果蝇睡眠作用的量效与时效关系。方法选取7日龄野生型Canton S品系黑腹果蝇为试验对象,在恒温、恒湿、自动光控条件下,利用自动红外果蝇活动监测系统,以果蝇24 h总睡眠时间和睡眠深度指数为观察指标,分别进行刺五加喷干粉改善果蝇睡眠作用的量-效与时-效关系的研究。结果雌、雄果蝇均在0.25%药物培养基、给药4 d以上的情况下,改善睡眠的作用最明显。结论刺五加喷干粉可以改善果蝇睡眠,并在一定范围内呈现量效和时效关系。

Objective To study the dose - effect and time - effect relationship of Ciwujia on sleep improvement in drosophila. Methods Under the standard conditions of constant temperature, humidity, automatically controlled lighting, an infrared drosophila activity monitor system （DAMS） was used to monitor the sleeping data of drosophila, and the dose - effect and time - effect of Ci- wujia on sleep improvement was studied in 7 - day - old drosophila. Results 0.25% drug medium for dose,≥4 days for delivery time was the best for female,and ≥5 days was the best for male. Conclusion Ciwujia spry - dried powder can improve sleep of drosophila in a certain dose range. The dose - effect and time - effect of Ciwujia on drophila sleep can be observed.