枸杞多糖对小鼠移植性肝癌端粒酶活性及hTERT蛋白表达的影响

The Effect of Lycium barbarum Polysaccharides on the Telomerase Activation and hTERT Expression on the Portability Liver Cancer in Mice

目的探索枸杞多糖(Lycium barbarum polysaccharides,LBP)对小鼠移植性肝癌端粒酶活性及其限速酶hTERT表达的影响。方法采用皮下接种法建立小鼠移植性肝癌模型,将96只小鼠随机分成正常对照组,模型组,LBP高、中、低(20,10,5 mg/kg·d)剂量组和环磷酰胺(20 mg/kg·d)组。观察LBP对小鼠肿瘤体积、抑瘤率的影响。PCR-TRAP-ELISA法检测端粒酶的活性,Western blot测定hTERT蛋白表达。结果 20,10,5 mg/kg·d的LBP能明显减小肿瘤细胞的体积和质量(P<0.05),抑瘤率分别为42.23%、25.10%和9.16%;同时,LBP(20,10mg/kg·d)能明显抑制肝癌组织hTERT蛋白表达(P<0.05),降低端粒酶的活性(P<0.05)。结论 LBP可通过抑制小鼠肝癌组织hTERT蛋白表达、降低端粒酶的活性,从而对小鼠移植性肝癌的生长起到抑制作用。

Objective To explore the effect of Lycium barbarum polysaccharides on the telomerase activation and hTERT expres- sion on the portability liver cancer in mice. Methods The portability liver cancer models were established with hypodermic inocu- lation. Ninety - six mice were randomly divided into 6 groups：normal control group, model group, LBP high, middle,low - dose group, positive control group. To observe the effect of LBP on the gross tumor volume, inhibition rates of tumor growth. Telomerase activation were determined by PCR -TRAP- ELISA, hTERT expression were determined by Western blot. Results LBP（20mg/ kg · d, 10mg/kg · d, 5mg/kg · d） could obviously depress the gross tumor volume and weight（P 〈 0.05）. The difference of in- hibition rates of tumor growth were 42.23% ,25.10% and 9.16qv. LBP（20mg/kg · d、10mg/kg · d） could obviously inhibit the hTERT expression and depress the telomerase activation in liver cancer （ P 〈 0.05 ）. Conclusion LBP can produce depressant effects on the portability liver cancer in mice. The effects concerned with the LBP can inhibit the hTERT expression and depress the telomerase activation.