摘要
以人参皂苷Re为标准对照,香草醛—冰醋酸—高氯酸作为显色体系,建立地瓜参皂苷含量测定方法,结果表明:皂苷浓度在40～200μg/mL时,与吸光度值呈良好线性关系,加标回收率为101.9%,RSD=4.63%,精密度试验RSD=1.77%,稳定性试验RSD=3.16%,重复性试验RSD=3.15%,说明方法学各指标均符合要求。
A quantitative analysis of the Acaudina molpadioides method was established by using the ginseng saponins Re as standard and vanillin—glacial acetic acid—high chlorine acid as color-forming reagents.The results showed that concentration had a good relationship with the absorbance when it was among 40-200 μg/mL.The average recovery was 101.9%,RSD=4.63%.Precision experiments RSD=1.77%,stability experiments RSD=3.16%,repetitive experiments RSD=3.15%,explained that the indexes of methodology met the requirements.
出处
《现代农业科技》
2012年第20期84-85,共2页
Modern Agricultural Science and Technology
关键词
海参
总皂苷
测定方法
sea cucumber
total saponin
determination
