胃溃疡、胃癌组织中幽门螺杆菌及真菌基因片段的扩增分析

Amplification Analysis of Helicobacter pylori and Fungi Gene Fragments in Human Gastric Ulcer and Gastric Cancer Tissues

探讨胃溃疡、胃癌组织中幽门螺杆菌(Helicobacter pylori,Hp)、真菌(Fungi)单纯感染及混合感染的可能性并进行验证。应用聚合酶链反应(PCR)技术,分别自4例胃溃疡和4例胃癌并伴单纯幽门螺杆菌、真菌及其混合感染病例石蜡包埋组织(FFPE)中扩增Hp及fungi基因特异片段并进行测序分析。成功提取了FF-PE胃组织基因组DNA,并扩增出Hp 16S rRNA及真菌内转录间隔区18S rDNA基因和28S rDNA之间的基因特异条带,测序大小分别为114 bp和357 bp,经在线BLAST比对分析表明所扩增基因与Hp及真菌核苷酸具有高度同源性。胃溃疡、胃癌组织中存在Hp和真菌单纯感染及混合感染。推测Hp与真菌混合感染可能是加重胃溃疡发展和诱发胃癌发生的又一致病因素。积极治疗Hp与真菌混合感染有助于提高胃溃疡的治愈率和减少胃癌的发生。

The possibility of simple and mixed infection with Helicobacter pylori（Hp） and fungi in human gastric ulcer and gastric cancer tissues was investigated,tested and verified.Specific gene fragments of Hp and fungi were amplified by polymerase chain reaction（PCR） and determined by sequencing in 8 FFPE specimens,including 4 gastric ulcer and 4 gastric cancer cases with accompanied infection by respective Hp and fungi simple and mixed Hp/fungi infection.Genomic DNA was successfully extracted from paraffin embedded tissues.Hp 16S rRNA gene and fungi ITS gene fragments were amplified by PCR.The size of the band was 114 bp and 375 bp respectively after sequencing.Comparison analysis through BLAST on line the amplified genes had high isogeny with Hp and fungal nulceotide.Therefore,simple and mixed Hp and fungi infection occurred in gastric ulcer and gastric cancer tissues,speculated that Hp/fungi mixed-infection might be another pathogenetic factors that aggravate gastric ulcer to develop and induce the occurrence of gastric cancer.Active treatment of Hp/fungi mixed-infection conduces to improve the cure rate of gastric ulcer and reduce the incidence of gastric cancer.