黄芪注射液联合葛根素注射液对2型糖尿病KKAy小鼠肾脏TGF-β_1和BMP-7表达的影响

Effects of Astragalus injection combined with puerarin injection on expression of BMP-7 and TGF-β_1 in type 2 diabetic KKAy mouse kidney

目的:观察黄芪注射液联用葛根素注射液对2型糖尿病KKAy小鼠肾脏转化生长因子β1(TGF-β1)和骨形态发生蛋白7(BMP-7)表达的影响。方法:雄性KKAy小鼠饲养至14周龄时随机分成模型组和黄芪注射液联合葛根素注射液治疗组,同龄雄性C57BL/6J小鼠作为正常组。观察各组小鼠一般状态并测量体重;检测20周龄、24周龄及28周龄小鼠空腹血糖(BG)、血清甘油三酯(TG)、胆固醇(TC)和血清肌酐(SCr);免疫组化法测定肾脏组织TGF-β1蛋白表达情况,RT-PCR法检测肾脏组织BMP-7和TGF-β1的mRNA表达情况。结果:与正常组相比,模型组小鼠的体重、BG、TG、TC和SCr均有较明显增高;肾组织TGF-β1蛋白和mRNA表达明显升高,BMP-7 mRNA表达下降。与模型组比较,黄芪注射液联合葛根素注射液治疗组小鼠的体重、BG、TG、TC及SCr均有不同程度下降;肾组织BMP-7 mRNA表达升高,而TGF-β1蛋白及mRNA表达明显下调。结论:黄芪注射液联合葛根素注射液对2型糖尿病KKAy小鼠肾脏有保护作用,其机制可能与恢复肾组织BMP-7表达,降低肾组织TGF-β1过度表达有关。

AIM： To observe the effects of Astragalus injection combined with puerarin injection on the expression of transforming growth factor beta 1 （TGF -β1 ） and bone morphogenetic protein 7 （BMP -7） in the kidney of type 2 diabetic KKAy mouse. METHODS： The male KKAy mice of 14 weeks old were randomly divided into model group and Astragalus injection combined with puerarin injection treatment （Astragalus ＋ puerarin） group. The age-matched male C57BL/6J mice were selected as normal group. The general conditions and body weight of the mice were observed. Blood glucose （BG）, triglyceride （TG）, cholesterol （TC） and serum creatinine （SCr） were examined at the 20th, 24th and 28th week. The protein expression of renal TGF - 131 was determined by immunohistochemical method. The mRNA expres- sion of BMP -7 and TGF - 13t was detected by RT - PCR. RESULTS： Compared with normal group, the body weight, BG, TG, TC and SCr increased significantly in model group. TGF - 131 expression at protein and mRNA levels was increased, while mRNA expression of BMP - 7 was decreased in KKAy miee. Compared with model group, the body weight, BG, TG, TC and SCr reduced in Astragalus ＋ puerarin group. The mRNA expression of BMP -7 in the renal tissues was higher, and TGF -β1 expression at mRNA and protein levels was significantly lower in Astragalus ＋ puerarin group than those in model group. CONCLUSION： Astragalus injection combined with puerarin injection has renal protective effects on type 2 diabetic KKAr mice. The mechanism may be related to restoring BMP -7 expression and reducing the overexpres- sion of TGF - β1 in renal tissues.