石墨炉原子吸收分光光度法直接测定全血中锗

Graphite furnace atomic absorption spectrophotometric method for the direct determination of germanium in blood

目的:建立石墨炉原子吸收分光光度法测定全血中锗的方法。方法:样品无需处理,以0.1%Tri-tonX-100/0.2%HNO3(v/v)为稀释液,以0.5 mg/ml硝酸钙为基体改进剂,对血液适当稀释后,直接用石墨炉原子吸收分光光度法测定锗的含量。结果:方法最低检出限(n=10)为0.75 mg/L,线性范围为0μg/L～190μg/L,相对标准偏差0.8%～3.2%,回收率为99.5%～103.6%。结论:方法具有简便、快速、回收率高、精密度高的优点,适用于全血中锗含量的测定。

Objective：To establish a graphite furnace atomic absorption spectrophotometric method for the deter- mination of germanium in whole blood. Methods： Without treatment, germanium content in the sample was directly determined by graphite furnace atomic absorption spectrophotometry after sample dilution with 0.1% TritonX - 100/ 0.2% HNO3 （v/v） as diluent and 0.5 mg/ml calcium nitrate as matrix modifier. Results： The detection limit of the method （ n = 10） was 0.75 mg/L, the linearity was observed in the range of 0 μg/L -90 μg/L, the relative stand- ard deviation was 0.8%- 3.2%, the recovery rate was 99.5% -103.6%. Conclusion： The method is simple, rapid with high recovery rate and precision, it is applicable to the determination of germanium content in whole blood.