高效液相色谱法测定猕猴桃中膨大素含量

Study on determination of expansin in kiwi by HPLC

目的:建立猕猴桃中膨大素的高效液相色谱测定法。方法:以甲醇-水(65∶35)为流动相,用HY-PERSIL C18(4.6 mm×150 mm,5μm)进行分离,于波长265 nm处检测。结果:线性范围为5μg/L～100μg/L(r=0.9999),回收率为98.5%,RSD为1.4%。结论:用该方法测定膨大素含量,结果稳定,重现性好,可操作性强,适于猕猴桃中膨大素含量的质量控制。

Objective：HPLC for determination of expansin in kiwi was established. Methods： An HYPERSIL C18（4.6 mm × 150 mm,5 μm） column was used for separation withmethanol- water（65： 35）as mobile phase and detected at 265 nm. Results ： The linear range was 5 μg/L - 100 μg/L （ r = 0. 9999 ） , and average recovery was 98.5% , and RSD was 1.4%. Conclusion： This method had the advantages of stable result, good reproducibility and strong operability, which was suitable for the determination of expansin in kiwi fruits.