乙肝患者血清大蛋白检测的临床意义

Clinical significance of serum large surface protein detection in the patients with hepatitis B

目的:通过检测乙型肝炎患者血清中HBV-DNA、乙型肝炎病毒外膜大蛋白(HBV-LP)、乙型肝炎病毒前S1抗原(Pre-S1)和乙肝病毒标志物,比较HBV-LP与Pre-S1的差异,探讨HBV-LP与乙肝病毒复制的关系。方法:对202例乙型肝炎患者和80例正常对照血清采用酶联免疫方法检测HBV-LP、Pre-S1以及HBV标志物,采用实时荧光定量PCR检测血清中HBV-DNA含量。结果:202例乙肝患者血清中HBV-LP阳性率为84.65%,与HBV-DNA阳性率为82.67%无显著性差异,明显高于Pre-S1的阳性率58.41%。同时在HBV-DNA阳性乙肝患者中,HBV-LP阳性率为94.61%,显著高于HBeAg的阳性率。HBV-DNA拷贝数的对数与HBV-LP含量具有相关性(r=0.928)。结论:HBV-LP与HBV-DNA相关性好,是能反映乙肝病毒复制的血清学指标。

Objective：To detect serum HBV -DNA, hepatitis B virus large envelope protein （HBV -LP）, hep- atitis B virus pre - S1 antigen （ Pre - S1 ） and hepatitis B virus markers in the patients with hepatitis B, compare the difference between HBV - LP and Pre - S1, and explore the relationship between HBV - LP and hepatitis B virus replication. Methods： HBV - LP, the Pre - S1 and HBV markers were mesured by enzyme - linked immunosorbent assay in 202 hepatitis B patients and 80 normal controls, and HBV - DNA content was mesured by real - time quan- titative PCR. Results： In 202 hepatitis B patients, the positive rate of HBV - LP（84.65% ） was higher than that of HBV -DNA（82.67% ） and Pre -SI （58..41%）, and no significant difference was found between the positive rates of HBV - LP and HBV - DNA. In hepatitis B patients with HBV - DNA positive, the positive rate of HBV - LP was 94.61%, obviously higher than that of HBeAg. The logarithm of copies of HBV - DNA was related to the content of HBV- LP（r = 0. 928 ）. Conclusion： There is close correlation between HBV- LP and HBV- DNA, HBV- LP can accurately reflect the HBV replication.