北京地区肺炎病例呼吸道病毒及肺炎支原体感染调查

Prevalence of human respiratory viruses and mycoplasma pneumoniae in pneumonia patients in Beijing

目的:调查北京地区肺炎病例中肺炎支原体及呼吸道病毒感染的病原学现况,探讨不同年龄组、不同月份病原学检出情况及其流行病学意义。方法:采用实时荧光PCR法及多重RT-PCR法,对501份呼吸道样本同时检测9种常见呼吸道病毒及肺炎支原体。结果:阳性检出率为40.32%,肺炎支原体、流感病毒、呼吸道合胞病毒、副流感病毒为主要病原体;不同年龄组的病原体检出阳性率不同;部分病原体有一定的季节分布规律。结论:肺炎支原体、流感病毒、呼吸道合胞病毒、副流感病毒为北京地区肺炎病例感染主要致病原,随年龄不同、流行季节不同而有一定的流行规律。

Objective： To study the situation of human respiratory viruses and Mycoplasma pneumoniae in pneu- monia patients in Beijing and to discuss the etiology and epidemiology significance in different age groups in different months. Methods： By real - time PCR and multiplex RT - PCR, 9 main kinds of respiratory viruses and Mycoplas- ma pneumoniae were detected in 501 clinical respiratory specimens. Results： The positive ratio was 40.32%. My- coplasma pneumoniae, influenza virus, respiratory syncytial virus, parairffluenza virus were the main pathogens. The positive ratio was different for each pathogen in different age groups. Some viruses showed regular seasonal distribu- tion feature. Conclusion： Mycoplasma pneumonia, influenza virus, respiratory syneytial virus, parainfluenza virus were the main pathogens leading to the pneumonia infection in Beijing and the pathogens have specific epidemiology characteristics, according to the different ages and seasons.