ELISA试验测定结核IgG抗体在肺结核诊断中应用价值的研究

The Study of Clinical Value of Detecting the Serum IgG Anti-tuberculous Antibody with ELISA in Diagnosis of Pulmonary Tuberculosis

用超声提取的结核菌H_(37)Ra株菌体蛋白为抗原,HRP-SAP为示踪物,将间接法ELISA方法标准化后,测定了483例血清结核IgG抗体的水平。本法较实用,检测的敏感性,特异性均满意,与痰菌检查,胸片检查法结合,可提高肺结核的确诊率。

After standardization of Indirect ELISA, microplate coated with ultrasonited protein antigen extracted from Mycobacterium H37Ra strain, blocking the remaining unbound sites on the plate with 10％ fetal bovine serum (treated with ammonium sulfate), indicative enzyme using HRP-SPA, we had determinated the level of IgG anti-tubercrlous antibody including 483 cases of serum specimens(157 cases from pulmonary tubercrlosis). The sensitivity was 0.792. the specificity was 0.954. The diagnosis accuracy of pulmonary tuberculosis group will be greatly increascd by making simultaneously three examination: tubercle bacillus finding, radiological signs, specific IgG antibody detection, the accuracy was 0.966. We believe that this diagnostic method will be useful in practice.