利用基因芯片分析糖尿病大鼠种植体周围骨组织的差异表达基因

Differential expression genes of bone tissues surrounding implants in diabetic rats by gene chip

目的:应用基因表达谱芯片对糖尿病与正常大鼠种植体周围骨组织的基因表达谱进行对比分析,并筛选差异基因。方法:将6只Wistar大鼠随机分为糖尿病组和正常组,糖尿病组采用链脲佐菌素(Streptozotocin,STZ)腹腔注射法建立糖尿病大鼠模型。分别在大鼠右侧胫骨骨骺端植入钛种植体,3个月后,截取种植体周围骨组织,提取总RNA,进行包括17983个基因的全基因组表达谱芯片检测,对差异表达基因进行GO分析,并采用实时定量PCR对芯片结果进行验证。结果:芯片结果显示,差异基因共1084个,其中糖尿病组表达增强352个,表达降低732个,GO分析涉及1154个不同的功能分类。结论:糖尿病大鼠种植体周围骨组织中与成骨相关的基因表达降低,与脂类代谢相关的基因表达升高。

PURPOSE： To compare mRNA expression profiles of bone tissues surrounding implants between normal rats and rats with diabetes using microarray technology.METHODS： Six Wistar rats were randomly selected and divided into normal model group and diabetic group.Diabetic model condition was established by injecting Streptozotocin into peritoneal space.Titanium implants were implanted into the epiphyseal end of the rats ＇ tibia.Bone tissues surrounding implant were harvested and sampled after 3 months to perform comprehensive RNA gene expression profiling,including 17983 for genome-wide association study.GO analysis was used to compare different gene expression and real-time PCR was used to confirm the results on core samples.RESULTS： The results indicated that there were 1084 differential gene expression.In the diabetic model,there were 352 enhanced expression genes,732 suppressed expression genes.GO analysis involved 1154 different functional type.CONCLUSIONS： Osteoblast related gene expressions in bone tissue samples of diabetic rats were decreased,and lipid metabolism pathway related gene expression was increased.