共刺激分子B7-H4在原发性干燥综合征患者唇腺组织和血清中的表达及意义

B7-H4 expression of salivary gland and sera in patients with primary Sjogren＇s syndrome

目的检测共刺激分子B7-H4在原发性干燥综合征（pSS）患者唇腺及血清中的表达及临床意义。方法选择2009年6月至2011年1月安徽医科大学附属省立医院风湿免疫科门诊和住院确诊的pSS患者40例，免疫组织化学染色和流式细胞术检测唇腺组织中B7-H4表达；酶联免疫吸附法检测pSS患者和健康对照血清中可溶性B7．H4的含量。结果免疫组织化学染色显示B7-H4表达在唇腺导管上皮细胞，pSS患者唇腺中B7-H4阳性导管数百分比为（42±21）％，疾病对照为（85±13）％，两者比较P〈0．05。流式细胞术表明pSS患者唇腺单细胞悬液中B7-H4的表达为（18±14）％，对照为（48±22）％，两者比较P〈0．01。另外，pSS患者血清可溶性B7-H4的含量（49±31）μg／L，比正常健康人群的含量（71±27）μg／L明显减少（P〈0．01），并且其和唾液流率、泪液流率明显正相关（P〈0．01）。结论B7-H4共刺激分子在pSS患者的唇腺组织及血清表达减少，提示其可能参与了pSS的疾病进展，但其具体机制尚需要进一步研究。

Objective To detect the expression of B7-H4 in salivary gland and sera in patients with primary Sjogren＇s syndrome （pSS）. Methods A total of 40 pSS patients were referred to our department from June 2009 to January 2011. Immunohistochemistry and flow cytometry were used to detect the expression of B7-H4 in salivary gland from pSS patients and disease controls. Enzyme-linked immunosorbent assay （ELISA） was used to detect soluble B7-H4 of serum from pSS patients and healthy donors. Results Immunohistological staining revealed that B7-H4 antigen was restricted to tubular epithelium. The B7-H4 positive expression of tubules in salivary gland biopsies from pSS patients （ 18 ±14） % were lower than that of controls （ 85 ±13 ） % （ P 〈 0. 05 ）. Flow cytometry revealed that the B7-H4 expression of cell suspensions from salivary gland from pSS patients （42 ±21 ）% were lower than that of controls （48 ±22 ）% （P 〈 0. 01 ）. And the serum level of soluble B7-H4 detected in pSS patients （49 ±31 ） μg/L significantly decreased than that in healthy donors （71 ±27 ） μg/L （ P 〈 0. 05 ） and positively correlated with saliva and tear flow rates （ P 〈 0. 01 ） respectively. Conclusion The expression of B7-H4 molecule may play some roles in the progression of pSS. And a further understanding of its mechanism helps to elucidate the pathogenesis of pSS.