高效液相色谱法同时测定苯妥英钠和卡马西平的血药浓度

Simultaneous Determination of Phenytion and Carbamazepine in Serum by HPLC

目的:建立了高效液相色谱法同时测定人血浆中苯妥英钠(DPH)、卡马西平(CBZ)的药物浓度。方法:以symmetryshieldRP18柱(5.0μm,3.9×150mm)为色谱柱。流动相为甲醇∶水为50:50,流速1.0mL.min-1。Waters2487双波长紫外检测器,检测波长210nm。苯乙酮为内标物。结果:线性范围为苯妥英钠(2.2～33.0)μg.mL-1r=0.9994;卡马西平(2.0～30.0)μg.mL-1,r=0.9998;平均回收率分别为97.5%、98.2%,高、中、低浓度样品的日间和日内测定相对标准偏差RSD<2%。结论:本法适用于苯妥英钠、卡马西平的血药浓度测定。

Objective： To establish an HPLC method for determining plasma level of Phenytoin, Carbamazepine. Methods： Acetophenone was selected as the internal standard.A symmetryshield RP 18 column （5μm, 3.9 × 150mm） was used. The mobile phase, methanol-phosphate solution（50：50） was used at a flow rate 1.0 mL·min-1, The detector is Waters 2487 Dual λ Absorbance Detector, detector was set at the wavelength of 210 nm. Results： The linear relation of DPH and CBZ were in the range of 2.2- 33.0 and 2.0-30.0ug/ml,respectively. The relative recoveries of DPH and CBZ were 97.5%, 98.2%, respectively. The RSD within-day and between-day were less than 2%. Conclusion： This method is suitable for therapeutic drug monitoring ofPhenytoin, Carbamazepine.