摘要
以草鱼为试验材料,测定不同条件下草鱼中内源性转谷氨酰胺酶(TGase)的活性。结果表明,草鱼糜中的TGase在pH8~9,25~45℃时表现出较高的活性,钙离子能激发其活性,DTT在低浓度(0~1mmol/L)范围时能增加它的活性,而NH4Cl、EDTA及一些金属离子抑制TGase的活性。
The characterization of transglutaminase (TGase) from grass carp was studied under different conditions The results showed that optimal pH and optimal temperature of transglutaminase from grass carp were 8-9 and 25-45 ℃ respectively. It could be activated by calcium ions. Dithiothreitol(DTT) can increase the TGase activity. However, NH4Cl, EDTA and some metal ions inhibit the activity of TGase from grass carp.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2012年第9期67-72,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
教育部新世纪人才项目(NECT-10-0733)
浙江省重点科技团队创新项目(2009R50036)
关键词
草鱼
转谷氨酰胺酶
鱼糜
特性
grass carp
transglutaminase
surimi
characterization