响应曲面法优化油茶粕抗菌肽制备条件

Optimization the Technology of Preparation of Antimicrobial Peptides Derived from Camellia Oleifera Abel Seed Meal Using Response Surface Methodology

以pepsin为水解酶,以对大肠杆菌的抑菌率为响应值,采用Design-Expert 7.1 Trial统计分析软件,设计4因素3水平试验,对油茶粕蛋白制备抗菌肽工艺进行优化。响应曲面法优化的试验结果:酶的添加量1.7%,pH1.9,反应温度36.8℃,反应时间60.10min。在此条件下,对大肠杆菌的抑制率为(66.05±0.22)%(n=4),与预测值67.88%无显著差异(P>0.05)。

In this paper, using inhibitory rate on E. coli as response and using pepsin as proteolytic enzyme, a 43 factorial central composite design （through Design-Expert 7.1.3 software） was employed to optimize the hydrolysis condi- tions for preparation of antimicrobial peptides derivated from Camellia Oleifera Abel seed meal protein. The best hydroly- sis conditions were as followings： the enzyme dosage 1.70%, pH value 1.9, reactional temperature 36.8 ℃, and reaction- al time 60.10min. Under these conditions, the inhibitory rate on E. coli was 66.05±0.22%（n=4）, without significant dif- ference with the predicted value 67.88%（P〉0.05）.