改性壳聚糖Cu(Ⅱ)配合物树脂对肌红蛋白的水解作用研究

Effect of Chemical Modified Chitosan Resin Complexed with Copper (Ⅱ) on Myoglobin Hydrolysis

以壳聚糖树脂和金属离子Cu(II)为原材料,制备可重复使用的改性壳聚糖Cu(II)配合物树脂(CMCR)。利用茚三酮比色法和SDS-PAGE电泳法研究CMCR对肌红蛋白(Mb)的催化水解作用。研究结果表明:在生理pH,60℃条件下,CMCR可高效催化水解Mb,24h后Mb的水解率在80%以上。CMCR负载Cu(II)具有很高的稳定性,1次水解反应后仍可保留98.17%的金属离子。CMCR本身具有很好的稳定性,重复使用4次后仍保持85%以上的催化水解活力。CMCR作为新型水解蛋白的介质,在食品等领域具有广泛的应用前景。

Chitosan and copper（Ⅱ） ion were used as primary materials to prepare chemical modified chitosan resin complexed with copper ion（CMCR）, which could be recycled. The hydrolyzing ability of CMCR was investigated through Ninhydrin Colorimetric method and SDS-PAGE electrophoresis method. The results demonstrated that myoglobin can be hydrolyzed more than 80% at physiological pH, 60 ℃ for 24 hours Copper ions chelated on the resin hold a desirable stability, more than 98.17% copper ions still complexed with the resin after the first reaction. CMCR could be reused for 4 times while the catalytic ability maintains 85% of the original. The results conformed that CMCR is a very promising medium for hydrolyzing protein in the area of food etc.