大鼠坐骨神经缺损后背根神经节组织lncRNA表达变化

Altered lncRNA expression following sciatic nerve resection in rats

目的:研究大鼠坐骨神经缺损后背根神经节中长链非编码RNA(long noncoding RNA,lncRNA)的表达变化。方法:建立SD大鼠坐骨神经损伤0天、1天、4天和7天模型,取背根神经节组织进行lncRNA检测,进行差异基因的筛选,并应用实时荧光定量逆转录-多聚酶链反应(qRT-PCR)对芯片结果进行验证;利用lncRNA与mRNA特异性表达的标准化信号强度,构建基因共表达网络。结果:共筛选出24个显著下调的lncRNAs,qRT-PCR检测的lncRNA AF130881和AB020616的表达变化与芯片一致。得到与lncRNA AF130881和AB020616正向或负向共表达的蛋白编码基因25个。结论:大鼠坐骨神经缺损后背根神经节组织中lncRNA的表达谱发生改变。

Objective： To analyze the expression of lncRNA in the dorsal root ganglia（DRGs） after resection of the sciatic nerve in rats.Methods： The expression of lncRNAs was examined in a sciatic nerve resection model at 0 d、1 d、4 d and 7 d by using an rat lncRNA array.The lncRNA expression was also confirmed by quantitative real-time polymerase chain reaction（qRT-PCR）.Gene coexpression network was constructed according to the normalized signal intensity of specific expression lncRNAs and mRNAs.Results： A total of 24 lncRNAs were identified which were significantly down-regulated in the DRGs after resection from 0 through 1 and 4 until 7 d.The expression of lncRNA AF130881 and AB020616 analyzed by qRT-PCR was in agreement with the microarray data.The network was constructed from lncRNA AF130881 and AB020616 positively or negatively coexpressed with 25 coding mRNAs at the serial time points.Conclusion： This study demonstrated a different involvement of lncRNAs in the DRGs after resection of the sciatic nerve in a rat model.