SCID小鼠原位接种LNCaP前列腺癌模型的建立

Establishment of the Orthotopic Implantation Model of the LNCaP Prostate Cancer Cell in SCID Mice

通过在SCID小鼠前列腺内接种LNCaP前列腺癌细胞建立了原位接种模型并探讨其应用价值。用微量注射器在SCID小鼠的前列腺左右背外侧叶包膜下接种小鼠前列腺癌LNCaP细胞106个建立原位移植瘤模型,以皮下移植瘤模型作为对照比较两者的生存期、肿瘤转移发生情况,并在电镜下观察LNCaP前列腺肿瘤细胞和小鼠正常前列腺组织细胞的特征。结果发现原位模型组小鼠平均生存时间为(21±2.1)d较对照组(35.0±4.5)d明显缩短,差异非常显著(P<0.01);原位模型组小鼠全部发生肿瘤转移,其中盆腔淋巴和肺的转移发生率分别为100%(15/15)、60%(9/15);电镜观察发现SCID小鼠的正常前列腺细胞与癌变后的细胞体积及细胞器均有很大差异。SCID小鼠的前列腺癌原位移植瘤模型较好地模拟了人癌体内的自然生长状况,为进行前列腺癌的研究提供有用的工具。

By the Vaccination of Prostate cancer cells in the Prostate of SCID mice, the paper establishes a Orthotopic model and discusses its value of application. Using the method of micro-syringe, the o.t. implantation model was estabhshed by subcapsulat implantation of 106 mice prostate cancer LNCaP cells into the prostate gland of LNCaP mice. The subcutaneous （ s.b. ） implantation model was used as control. The survival time and the rate of metastasis of these two models were compared. Also the transmission electron microscope was used to observe the difference between the SCID＇s normal prostate cells and LNCaP cells. The survival lime of o.t. model was （ 21 ± 2.1 ）clays, which was markedly shorter than that of s.b. model （ 35.0 ± 4.5 ） days （ P〈0.01 ） . Metastasis occurred in all mice of the o.t. model with the highest rate of pelvic mode （ 100% ） and the lung （ 60% ） . No metastasis was seen in the control group except local growth and infiltration. The result s of transmission electron microscope showed the two types of cells had significant difference in the volume and their organelles. The mice prostate cancer cell lines LNCaP o.t. implantation model is an optimal clinical animal model to observe metastasis the mice natural situation of prostate cancer in its baby quite well.