猪皮胶原蛋白水解物体外抗氧化作用模式初探

Antioxidant Activity and the Possible Mechanism Pig Skin Collagen Hydrolysates

通过测定不同浓度不同水解时间的猪皮胶原蛋白水解物的还原能力、清除自由基能力、抗脂质氧化能力、金属离子鳌合能力来探讨其体外抗氧化作用机制。结果表明:在一定水解范围内,猪皮胶原蛋白水解物的抗氧化活性随着底物浓度的增加而增加,且水解物的还原能力、自由基清除能力、抗脂质氧化能力及Cu2+和Fe2+鳌合能力均随水解时间的延长而增大;底物浓度为4%、水解6 h的猪皮胶原蛋白水解物与其他水解条件下的水解物相比,具有较高的抗脂质氧化能力和较强的清除自由基能力,且具有丁基羟基甲苯(butylated hydroxytolu-ene,BHT)、抗坏血酸(ascorbic acid,Vc)等抗氧化剂所不具备的金属鳌合能力;4%的猪皮胶原蛋白6h水解物与0.02%Vc的还原能力相近。猪皮胶原蛋白水解物具有较高的抗氧化活性,可以作为氢供体、自由基稳定剂和金属离子螯合剂来抑制脂肪氧化。

In order to analysis antioxidant activities of pigskin collagen hydrolysate comprehensively, the reduc- tion capacity, scavenging free radical capacity, lipid oxidative capacity, metal chelating ability of pig skin collagen hydrolysate with different hydrolysis time and concentration were studied. Pig skin collagen hydrolysates possessed re- markable reducing power and strong radical-scavenging ability, and both were increased with hydrolysis time. The protein hydrolysates also showed strong Cu2＋ and Fe2＋ chelation ability, and the antioxidant activity increased with substrate concentration. The reducing power of 4% pigskin collagen with 6h hydrolyzing was similar to those of 0.02% ascorbic acid. The results demonstrated that enzyme-hydrolyzed pig skin collagen can act as a hydrogen do- nor, a radical stabilizer, as well as a metal ion chelator to inhibit lipid oxidation.