四种无机盐对南方根结线虫卵囊、卵孵化及其幼虫存活的影响

Effects of inorganic salts on eggs,egg masses hatching and 2nd instar larval survival of Meloidogyne incognita

为了明确NH4HCO3、CuSO4.5H2O、CsCl、NH4NO34种无机盐对南方根结线虫卵囊、卵孵化及其幼虫存活的影响。在室内条件下,采用浸渍法测试了4种无机盐在不同浓度和时间处理的作用效果。结果显示:各种无机盐均对线虫卵、卵囊孵化有抑制作用,对2龄幼虫有致死作用。随着无机盐浓度的增加和处理时间的延长,线虫卵、卵囊孵化的抑制率和2龄幼虫的死亡率显著提高。其中0.05 mol/L NH4HCO3对线虫卵囊孵化的抑制效果最好,处理16 h达81.17%,24 h后达100%;对线虫卵孵化也有很强的抑制作用,处理16 h达93.33%,24 h后达100%。各种无机盐在不同浓度和处理时间内均对2龄幼虫有致死效果,且半致死中浓度LC50不同,其中CuSO4.5H2O的LC50最小,NH4HCO3和CsCl次之。表明CuSO4.5H2O对南方根结线虫2龄幼虫有较强的致死活性,南方根结线虫2龄幼虫对CsCl的敏感性较高。

In order to determine the effects of NH4HCO3, CuSO4 · 5H2O, CsCl and NH4NO3 on eggs, egg masses hatching and 2nd instar larval （J2） survival of Meloidogyrte incognita, these salts with differ- ent concentrations and time were tested in the laboratory using dipping method. The results showed that inorganic salts had strong inhibition to eggs, egg masses hatching and lethal to J2. The hatching ratio and J2 mortality were significantly enhanced with the increase of treating concentrations and time. 0.05 mol/L NH4HCO3 demonstrated the highest inhibition effect on egg masses hatching with the inhibition ratio 81.17% after 16h and 100% after 24h, respectively. 0.05 moL/L NH4HCO3 also showed the high inhi- bition effect to egg hatching with 93.33% inhibition ratio treated after 16 h and 100% after 24 h. Inor- ganic saks with different concentrations and time had lethal effects to J2 and had different LCso. CuSQ . 5H20 showed the smallest LC50, and NH4HCO3 and CsC1 the second. These results demonstrated thatCuSO4· 5H2O had the strongest lethal effect to J2 of M. incognita. And 32 of M. incognita had the highest sensitivity to CsCl.