摘要
本研究结合全基因组关联分析结果筛选出3个与鸡蛋品质相关的候选基因,分别为组织蛋白酶B基因(CTSB)、H-钙黏蛋白13基因(CDH13)和胞外吡啶核苷酸氧化酶二硫键巯基交换酶基因(ENOX1),进而研究其RNA表达情况是否与表型性状之间存在关联。在"农大3号"矮小型褐壳蛋鸡群体中,这3个基因无论是SNP基因分型还是表型值分类,其表达水平与相应的性状都没有显著相关。但在白来航鸡群体中与蛋壳比例对应的基因CTSB在按表型分类时,表达量差异接近显著(P=0.05),且两者相关达到显著水平。
This study was aimed to test whether gene expression level of selected three genes was significantly associated with egg quality traits. Three genes （cathepsin B,H-cad protein 13 and ecto NOX disulfide thiol exchanger 1） from previous genome-wide association study were selected for expression analysis, which had been considered to be related with egg quality traits. The mRNA expression differences had not been observed in different genotypes or phenotypes of brown-egg dwarf layer population. The expression difference of cathepsin B gene corresponding to eggshell proportion was marginal significant （P=0.05）in white Leghorns population. Interestingly,the expression level was negatively correlated with eggshell proportion value （r=-0.62）.
出处
《中国家禽》
北大核心
2012年第20期10-13,共4页
China Poultry
基金
国家蛋鸡产业技术体系专项资金(CARS-41)
中央高校基本科研业务费(2011JS003)
