PLC_ε基因敲除小鼠微卫星DNA遗传监测分析

Genetic Monitoring of PLC_ε Knockout Mice by Microsatellite DNA Analysis

目的利用多态性微卫星DNA位点分析PLCε基因敲除小鼠的遗传特性。方法用所筛选的15个微卫星DNA位点对28只PLCε基因敲除小鼠的DNA进行了PCR扩增,通过基因片段大小来分析群体的遗传多样性。结果 13个微卫星DNA位点中(D1Mit365、D3Mit51、D4Mit235、D6Mit102、D7Mit281、D8Mit113、D9Mit23、D10Mit180、D13Mit88、D16Mit145、D17Mit36、D18Mit94、D19Mit97)每个位点的28只小鼠DNA片段泳动距离一致,呈现单态性,表明该群体符合近交系的遗传特性;而利用Dq(敲基因型)和Dy(野生型)两个位点对28只小鼠的PCR扩增结果进行了鉴别分析,其中敲除基因型小鼠为6只;野生型为7只;杂合型为15只。结论利用微卫星标记技术可以对群体进行遗传质量监测,并能有效地鉴别不同的基因型,为小鼠的遗传质量监测提供了一种可行的方法。

Objective To determine the genetic characteristics of PLCε knockout mice by polymorphic microsatellite DNA loci analysis.Methods Genome DNA of 28 PLCε gene knockout mice were amplified by PCR using screening 15 microsatellite DNA loci,and population genetic diversity was identified by gene fragments.Results Among 13 microsatellite DNA loci（D1Mit365,D3Mit51,D4Mit235,D6Mit102,D7Mit281,D8Mit113,D9Mit23,D10Mit180,D13Mit88,D16Mit145,D17Mit36,D18Mit94,D19Mit97）,each locus of electrophoresis distance of DNA fragments in the 28 PLCε gene knockout mice kept consistent and presented monomorphism,indicating the genetic stability.However the two loci Dq（knock genotype） and Dy（wild-type） were used to discriminate 28 PLCε gene knockout mice by PCR amplification.Among them,6 mice were of gene knock mice,7 mice were of wild-type mice,and 15 mice were of heterozygous type.Conclusions The method of microsatellite marker analysis can be used to monitor population genetic quality and accurately distinguish different genotypes of mice,providing a feasible method for the detection of genetic quality in mice.