中国荷斯坦奶牛乳腺炎相关基因IRAK2基因诊断方法的研究

Study on genetic Diagnosis for IRAK2 gene associated with mastitis in Chinese Holstein cattle

为了研究奶牛乳腺炎相关基因IRAK2第7外显子SNP基因多态性,试验根据GenBank中IRAK2基因序列设计引物,PCR扩增得到281 bp和279 bp的IRAK2基因片段,将所得的片段经PCR-SSCP方法和RsaⅠ内切酶酶切检测后均出现3种基因型。结果表明:获得的基因型与NCBI中报道的一致。说明用PCR-SSCP联合酶切方法建立IRAK2基因快速诊断方法是可行的。

To investigate the single nucleotide polymorphism （SNP） in exon 7 of IRAK2 gene associated with mastitis, the primers were designed based on the sequences of IRAK2 gene published in GenBank, and the 281 bp and 279 bp fragments were obtained by PCR amplifica- tion. The fragments were deteeted by using PCR - SSCP mad Rsa I restriction enzyme, and then the three genotypes were founded. The results showed that Ih, three genotypes were consistent with those published by NCBI. It indicates that it is feasible to establish a rapid diagnostic meth- od for IRAK2 gene by using PCR - SSCP combined with the restriction endonuclease.