大黄提取物对成年大鼠睾丸的毒性作用

Toxicity of Rheum extracts on testes of adult rats

目的探讨长期应用大黄及其总蒽醌对雄性大鼠生殖功能的影响。方法 8周龄SD大鼠50只,分别ig给予大黄水提取物(RWE)0.3,0.6,1.2 g.kg-1及大黄总蒽醌0.1 g.kg-1,每天1次,连续30 d。取睾丸、附睾称质量,检测精子数量、活动率及畸变率。ELISA法检测血清中睾酮(T)、黄体生成素(LH)和卵泡刺激素(FSH);免疫组化SP法检测睾丸Bcl-2,Bax蛋白的表达水平。结果与正常对照组比较,RWE0.3,0.6和1.2 g.kg-1组大鼠精子数量和活动率均明显降低(P<0.05),精子畸变率无统计学变化;与正常对照组比较,RWE 0.6和1.2 g.kg-1以及大黄蒽醌0.1 g.kg-1组血清中T由5.0±0.7降低到2.6±0.4,1.5±0.5和(1.2±0.3)μg.L-1(P<0.05),LH由4.3±0.4升高到6.9±0.5,10.1±1.0和(10.5±0.8)μmol.min-1.L-1及FSH由2.7±0.5升高到6.3±1.2,11.3±0.8和(12.0±1.2)μmol.min-1.L-1(P<0.05);与正常对照组比较,RWE 0.6和1.2 g.kg-1以及大黄蒽醌0.1 g.kg-1组睾丸生精上皮和睾丸间质细胞内Bax蛋白表达率由12±3升高到35±4,55±6和(57±5)%(P<0.01),而Bcl-2蛋白表达率由60±4降低到31±4,8±4和(8±4)%(P<0.05)。结论长期应用大黄对成年大鼠睾丸功能有较强的毒性。

OBJECTIVE To investigate the effect of Rheum water extracts（RWE） and total anthraquinone（RTA） on the reproductive function of adult male rats.METHODS Fifty SD rats were ig given RWE 0.3,0.6 and 1.2 g.kg-1 and RTA 0.1 g.kg-1,once daily,for 30 d.Then,testes and epididymis were taken out,sperm count was detected,and the rate of sperm mobility and aberration was determined.The level of testosterone（T）,luteinizing hormene（LH）,and folliclestimulating hormone（FSH） in serum and the expression of Bcl-2 and Bax in testes were measured by ELISA and immunohistochemistry S-P method,respectively.RESULTS Compared with normal control group,the sperm count,sperm mobility rate in RWE 0.3,0.6,1.2 g.kg-1 and RTA 0.1 g.kg-1 groups significantly decreased（P 0.05） while the malformation rate showed no significant change.Compared with control group,T level in RWE 0.6 and 1.2 g.kg-1 and RTA 0.1 g.kg-1 groups significantly decreased from 5.0 ± 0.7 to 2.6 ± 0.4,1.5 ± 0.5 and（1.2 ± 0.3） μg.L-1,respectively（P 0.05） while LH significantly increased from 4.3 ± 0.4 to 6.9 ± 0.5,10.1 ± 1.0 and（10.5 ± 0.8） μmol.min-1.L-1,respectively（P 0.05）,and FSH from 2.7 ± 0.5 to 6.3 ± 1.2,11.3 ± 0.8 and（12.0 ± 1.2） μmol.min-1.L-1,respectively（P 0.05）.Compared with normal control group,the positive expression rate of Bax significantly increased from 12 ± 3 to 35 ± 4,55 ± 6 and（57 ± 5） %（P 0.01）,but the positive expression rate of Bcl-2 significantly decreased from 60 ± 4 to 31 ± 4,8 ± 4 and（8 ± 4） %（P 0.05） in RWE 0.6 and 1.2 g.kg-1 and RTA 0.1 g.kg-1 groups.CONCLUSION Rheum has toxic action on reproduction function.