雄激素受体和转化生长因子-β1在大鼠多囊卵巢综合征中的作用

Efficacy of AR and TGF-β1 in the pathogenesy of rat＇ s polycystic ovarian syndrome

目的从整体水平、分子水平综合分析雄激素受体（AR）及转化生长因子-β1（TGF-β1）在多囊卵巢综合征（PCOS）发病机制中的作用。方法本研究通过建立来曲唑所致PCOS大鼠模型，其中实验组灌胃服用来曲唑1mg／（kg·d），溶于1％羧甲基纤维素中，实验对照组灌胃服用1％羧甲基纤维素，空白对照组不给予灌胃刺激。连续灌服21d。应用Wester Blot方法观察AR及TGF—β1在子宫内膜中的表达。结果实验组AR水平明显低于实验对照组及空白对照组（P〈0．05），实验组TGF-β1水平明显高于实验对照组及空白对照组（P〈0．05）。结论AR、TGF—β1在子宫内膜中的表达及相互作用与PCOS的发病密切相关。

Objective To analyse the effect of AR and TGF-β1 in the pathogenesy of polycystic ovarian syndrome （PCOS） from the whole level and the molecular level. Methods To set up a rat＇ s model of PCOS using letrozole. The treatment group was administered letrozole at concentration of 1 mg/ （ kg . d）, P.O. Dissolved in 1% aqueous solution of carboxmethlycellulose （ CMC ） once daily, while normal control group received vehicle only 1% CMC once daily orally and blank control group received nothing. The treatment period was 21 days, and investigate the expression of TGF-β1 and AR in the endometrium through the method of Western Blot, so as to analyse how they act on the pathogenesy of PCOS. Results The results showed various expression of AR and TGF-β1 in the endometrium ： The level of AR in treatment group was more lower than that in control group （ P 〈 0. 05 ）, while the level of TGF- β1 was more higher（ P 〈 0. 05 ）. Conclusions The expression of AR and TGF-β1 and the coopration of them in the endometrium is highly correlated with the pathogenesy of PCOS.