Evi-1及c-myb基因与胃癌超声内镜分期及病理分级的相关性研究

Correlation of expression of both Evi-1 and c-myb genes with endoscopic ultrasolography stage and pathologic differentiated grade in gastric adenocarcinoma

目的通过对胃癌组织中Evi-1及c-myb基因的检测,探讨2个基因在胃癌中的表达水平,及其与超声内镜下胃癌分期、病理分化程度的相关性。方法经内镜取活检,病理检查确诊分为低分化腺癌、中分化腺癌、高分化腺癌。超声内镜下胃癌浸润深度T分期为T1期、T2期、T3期、T4期,用实时定量荧光PCR法(Real-time)检测Evi-1及c-myb基因在不同病理分化程度及不同超声内镜分期癌组织、萎缩性胃炎组织及正常组织中的表达。结果 Evi-1基因在胃癌组织中高表达,在正常对照组及萎缩性胃炎中低表达。Evi-1基因的表达在胃癌组与正常对照组比较(P=0.00)、胃癌组与萎缩性胃炎组(P=0.018)比较差异均有显著性;Evi-1基因在转移组与非转移组比较(P=0.015)差异有显著性。Evi-1基因表达与病理分化程度(P=0.70)及超声内镜分期比较(P=0.46)差异无显著性。c-myb基因在3组均表达,胃癌组与正常对照组(P=0.29)及萎缩性胃炎组(P=0.50)比较差异无显著性。结论 Evi-1基因在胃癌组织中高表达,淋巴结转移组中该基因表达明显增高,但其表达与病理分化程度及超声內镜分期差异无显著性;Evi-1基因表达与胃癌转移有关,有望成为胃癌的诊断指标,并判断胃癌的预后,为进一步靶向治疗提供依据。

Abstract： [ Objective ] To explore the expression level of Evi-1 and c-myb genes, and investigate their correla- tion of expression level with endoscopic, ultrasonography stages and pathologic differentiated grades in gastric ade- nocarcinoma. [Methods ] Gastric adenocarcinoma was diagnosed under gastroscopy, then T1, T2, T3, T4 stages were evaluated under endoscopic Ultrasonography and poorly, moderately, well differentiated grades by pathologist. There were cancer, control and atrophic gastritis groups. The Evi-1 and c-myb genes were demonstrated by the real-time fluorescence Polymerase Chain Reaction technique. [ Results ] The Evi-1 gene was high expression in cancer group compared with atrophic gastritis group （P =0.018） and control group （P =0.00）, and low expression in atrophic gastri- tis group and control group, and high expression metastasis group （P =0.015） compared with no metastasis one. Itwas no statistically evaluated in pathologic differentiated grades （P =0.70） and Endoscopic Uhrasonography stages （P =0.46）. The c-myb gene was expressed in three groups. Its expression was no statistically evaluated in cancer group compared with control （P =0.29） and atrophic gastritis groups （P =0.50）. [ Conclusions] The Evi-1 gene was high level expression in cancer group, especially in metastasis group, however, the c-myb gene was expression in all groups. Both of genes were not associated with endoscopic Uhrasonography stages and pathologic differentiated grades in gastric carcinoma. It is suggest that Evi-1 genes can be regarded as sign for diagnosis and predict progno- sis of gastric carcinoma, and suggested a target for treating gastric adenocarcinoma.