经程序化冷冻的小鼠休眠胚胎的基因表达谱差异分析

Gene expression profile analysis of dormant mouse embryos preserved by controlled slow freezing

目的探讨小鼠休眠胚胎经程序化冷冻后基因表达谱的变化及相关信号通路的改变趋势。方法采用Affymetrix基因芯片检测小鼠正常休眠胚胎和经程序化冷冻后的休眠胚胎的差异表达基因;采用GO分析和Pathway分析等生物信息学方法进一步了解相关信号通路的改变。结果经程序化冷冻后的小鼠休眠胚胎与正常休眠胚胎相比,存在228个差异表达基因,其中50个基因表达上调,178个基因表达下调。Pathway分析显示黏着斑通路、细胞外基质受体相互作用通路、肌动蛋白细胞骨架调节通路、细胞凋亡通路、细胞通讯通路、泛素介导的蛋白质水解通路、甘油磷脂代谢通路、小细胞肺癌通路、TGF-β信号通路、MAPK信号通路等基因差异表达变化趋势明显。结论小鼠休眠胚胎经程序化冷冻后会导致一系列基因调控变化,并可能影响多条信号通路的协同变化。

Objective To investigate the difference of the gene expression profiles in dormant mouse embryos preserved by controlled slow freezing.Methods The differences between gene expression profiles of normal dormant mouse embryos and those preserved by controlled slow freezing were detected by Affymetrix Gene Chip detection.GO bioinformatics and pathway analysis were also applied for further understanding the potential changes of their signaling pathways.Results After the treatment by controlled slow freezing,a total of 228 genes were changed between these two groups.Among them,50 genes were up-regulated,and other 178 ones were down-regulated.In addition,the signal pathway analysis revealed that the focal adhesion,ECM-receptor interaction,regulation of actin cytoskeleton,apoptosis,cell communication,ubiquitin-mediated proteolysis,glycerolphospholipid metabolism,small cell lung cancer signaling,TGF-beta signaling,and MAPK signaling pathways were significantly changed.Conclusions The results of our study indicate that controlled slow freezing leads to a series of potential changes of gene regulation and signaling pathways in dormant mouse embryos.