人类与部分实验动物RETN基因同源性分析

Analysis of resistin gene homology of humans and some laboratory animals

目的分析人类与部分实验动物的RETN基因同源性,为人类RETN基因相关疾病及基因功能研究对理想实验动物的选择提供依据。方法提取猕猴、大鼠、小鼠和树鼩的脂肪组织总RNA,用相应的RETN引物进行RT-PCR,对其产物进行双向测序;同时在GenBank中查询人类、猪、牛的RETN基因序列;再运用ORF Finder软件调取其ORF核苷酸序列和氨基酸序列,用DANMAN软件对所获得的序列进行比对,分析其同源性。结果人类RETN的核苷酸序列与猕猴、小鼠、树鼩、大鼠、猪、牛的同源性分别为95.4%、65.4%、65.4%、66.4%、81.0%、81.0%;氨基酸序列分别为91.7%、55.6%、55.6%、53.7%、75.9%、72.2%。;系统进化树结果表明,就RETN基因而言,人与猕猴的亲缘关系较近。结论基于同源性分析结果,在RETN的进化上猕猴与人类存在较近的亲缘关系,是研究人类RETN生物学功能及其相关疾病的最佳实验动物。

Objective To analyze the resistin gene homology of humans and some laboratory animals,and provide a basis for the selection of ideal laboratory animals to carry out human disease and gene function studies.Methods To extract total RNA of adipose tissue from macaques,rats,mice and tree shrews.The RT-PCR products were analyzed with bi-directional sequencing.The sequences of resistin gene of human,pig and cattle were inquired in GenBank.Their ORF nucleotide and amino acid sequences were transfered to use the ORF Finder software,and analyzed their sequence homology.Results Human resistin gene of nucleotide sequence homology was 95.4% with macaques,65.4% with mice,65.4% with tree shrews,66.4% with rats,81.0% with pigs and 81.0% with cattles.The amino acid sequence was 91.7% with macaques,55.6% with mice,55.6% with tree shrews,53.7% with rats,75.9% with pigs and 72.2% with cattles.The phylogenetic tree results showed a closer relationship between humans and macaques.Conclusions Based on the resistin gene homology analysis results,it was found that macaques have a closer relationship with humans,and are best laboratory animals in studies of human resistin biological function and related diseases.