同步荧光法测定烤鱼片中的多环芳烃

Determination of Polycyclic Aromatic Hydrocarbons in Baked Fish by Synchronous Fluorescence Spectrometry

建立了烤鱼片中多环芳烃(PAHs)的同步荧光测定方法。样品经甲醇-氢氧化钾皂化,环己烷提取;提取液经硅胶柱净化、浓缩,同步荧光光谱法测定其PAHs种类及含量。PAHs回收率81.3%～100.6%,相对标准偏差0.78%～1.13%。该方法灵敏度高、准确度好,能同时测定多种PAHs,适合于烤鱼片中PAHs的分析测定。

Determination of polycyclic aromatic hydrocarbons in baked fillet was established by synchronous fluorescence spectroscopy.The samples were extracted with cyclohexane after methanol-potassium hydroxide saponification and cleaned up by the columns of neutral silica gel.7 PAHs in the samples were separated and quantified by the proposed method.The recoveries of the method ranged from 81.3% to 100.6%,and the relative standard deviations were 0.78% to 1.13% This method is sensitive,accurate and suitable to determinate PAHs in baked fish.