花叶玉簪试管苗快速增殖与移栽

Rapid Proliferation and Transplant of Plantlets in Hosta plantaginea

利用花叶玉簪试管苗进行其快速繁殖、壮苗生根与移栽实验,结果表明,在MS基本培养基中附加低浓度6-BA(0.1～0.5 mg/L)和生长素(IBA 0.01 mg/L或NAA 0.1 mg/L)有利于花叶玉簪试管苗离体分株;而附加高浓度6-BA(≥1.0 mg/L)和生长素(IBA 0.01 mg/L或NAA0.1 mg/L)则有利于花叶玉簪愈伤组织分化产生不定芽。MS培养基附加低浓度6-BA(0.1～0.5mg/L)或不加6-BA,配合一定浓度的生长素(IBA 0.1～0.3 mg/L或NAA 0.1 mg/L)有利于花叶玉簪试管苗壮苗生根。生根良好的花叶玉簪试管苗移栽成活率达91%±2%。

In this paper,plantlets of Hosta plantaginea were used as explants to investigate the proper conditions for rapid proliferation,growth of plantlets,rooting and transplanting.The results showed that MS medium supplemented with 6-BA（0.1-0.5 mg/L） and IBA（0.01 mg/L） or NAA（0.1 mg/L） were suitable for proliferation of plantlets via in vitro ramet,while MS medium supplemented with 6-BA（≥1.0 mg/L） and IBA（0.01 mg/L） or NAA（0.1 mg/L） were suitable for bud differentiation induced from calli.MS medium supplemented with IBA（0.1-0.3 mg/L） or NAA（0.1 mg/L）,with or without low concentrations of 6-BA（0.5 mg/L） were suitable for the growth and rooting of plantlets.Finally,a total survival rate of 91%±2% was obtained for transplanting.