超高效液相色谱法测定卷烟包装纸中的甲醛和乙醛

Determination of Formaldehyde and Acetaldehyde in Cigarette Wrapping Paper by UPLC

研究了超高效液相色谱用于卷烟包装纸中甲醛和乙醛含量测定方法。甲醛和乙醛通过2,4-二硝基苯肼衍生化后,以0.5%甲酸水溶液-乙腈(体积比为65∶35)为流动相,在Waters BEHShield RP18(1.7μm,2.1 mm×50 mm)色谱柱上分离,二极管阵列检测器检测,检测波长360 nm。甲醛和乙醛在5 min内即可完全分离,方法具有较好的重现性和线性关系,相关系数均达到0.999 9,加标回收率甲醛为97.9%,乙醛为95.5%;用于卷烟包装纸中甲醛和乙醛的快速测定,结果满意。

An ultra performance liquid chromatographic （UPLC） method for determination of formaldehyde and acetaldehyde in cigarette wrapping paper was studied. The formaldehyde and acetaldehyde were derivatizated with 2,4-dinitrophenylhydrazine, then they were separated by Waters BEH ShieldRP18 column with 0.5% formic acid and acetonitrile （65：35, v/v） as mobile phase and detected with photodiode array detecter （PDA） at 360 nm. The formaldehyde and acetaldehyde were completely separated and detected in 5 min. The method has good repeatability and linearity. All the correlation coefficients are above 0. 9999. Their recoveries were 97.9% and 95.5% respectively. The method was satisfied with the results by rapid determination of formaldehyde and acetaldehyde in cigarette wrapping paper.