马铃薯愈伤组织再生体系的研究进展

Research Progress on Callus Regeneration System of Potato

马铃薯愈伤组织再生体系的建立是进行马铃薯遗传转化的基础。外植体选择、培养基配方以及培养过程中褐化的控制等都是影响愈伤组织诱导和再生体系建立的关键因素。本文主要从愈伤组织诱导、愈伤组织继代和分化三个阶段进行详细总结,并提出愈伤组织诱导,建立再生体系中存在的问题及今后的研究方向,以期为建立马铃薯愈伤组织再生体系提供理论参考和技术借鉴。

Callus regeneration system of potato was established,which was the base for potato genetic transformation.Explant selection,medium formula and browning control of culture process are key factors influencing the callus inducement and the regeneration system establishment.This paper summarized the three phase of callus inducement,subculture and differentiation.Itraisedsomequestionsandgavefutureresearchdirectionaboutthecallus inducement andestablishment of callus regenerationsystem.Itwouldprovidetheoreticalandtechnicalreferencesforestablishingthecallusregenerationsystem ofpotato.