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SOCS1-siRNA沉默SOCS1基因表达对脐血树突状细胞生物学活性的影响 被引量：2

The effects on the biological activity after silencing of SOCS1 gene expression on cord blood dendritic cell with SOCS1-siRNA
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摘要 目的采用RNA干扰技术抑制脐血树突状细胞(DC)SOCS1基因表达,并检测其对树突状细胞生物学活性的影响,为树突状细胞的临床应用奠定基础。方法针对SOCS1基因,采用化学合成法合成SOCS1siRNA,并转染脐血DC。RT-PCR和Western blot检测转染前后DC的SOCS1基因表达;流式细胞术检测细胞表面CD80、CD86及HLA-DR分子表达情况;ELISA法检测细胞培养上清中IL-12水平。结果与对照组相比,siRNA组SOCS1蛋白质表达水平降低,差异有统计学意义;DC表面CD80、CD86及HLA-DR分子表达上调,DC培养上清中IL-12的浓度显著提高。结论 RNA干扰技术能显著下调脐血树突状细胞SOCS1的表达,为以DC SOCS1为靶向的抗肿瘤治疗提供了新思路和新手段。 Objective Inhibited the expression of SOCS1 gene on cord blood dendritic cell（DC） by RNA interference and detected the biological activities of DC.Methods The SOCS1 siRNA were synthesised and transfected into cord blood dendritic cell.The expression of SOCS1 gene on DC before and/or after transfected with siRNA sequence was evaluated by RT-PCR and Western blot assays.The phenotype of DC were detected by flow cytometry.The concentration of lL-12 in the supernatant was detected by ELISA analysis.Results Compared with the control group,SOCS1 gene expression were significantly reduced in siRNA group.RNA interference increased the expression of CD80,CD86,and HLA-DR on DC and promoted the concentration of IL-12 in the supernatant.Conclusion RNA interference can significantly reduce the SOCS1 expression on DC.This research provided a new approach to the clinical application of dendritic cells.
作者 于鸿 陈阳 张健 徐广伟
机构地区 吉林省肿瘤防治研究所 吉林大学第三医院
出处 《中国实验诊断学》 2012年第10期1794-1796,共3页 Chinese Journal of Laboratory Diagnosis
基金 吉林省卫生厅课题(2010Z036)
关键词 树突状细胞 SOCS1 RNA干扰 dendritic cell SOCS1 RNA interference
分类号 R392.1 [医药卫生—免疫学]
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参考文献9

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二级参考文献44

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共引文献8

同被引文献37

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中国实验诊断学
2012年 第10期

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