观赏海棠不同组织中RNA的几种提取方法

Methods of RNA Isolation from Different Organizations of Malus Crabapple

以观赏海棠不同组织为试材,用常用的CTAB法、异硫氰酸胍法及Tris-硼酸法,比较其提取RNA的质量和纯度。结果表明:CTAB法提取RNA成本低、RNA质量较高;异硫氰酸胍法提取RNA的质量好、纯度高、提取效率高;而Tris-硼酸法则较不适合于果实类多酚多糖组织的RNA提取。

CTAB method, Guanidine thiocyanate method and Tris-- borate method were employed to isolate total RNA from dif- ferent organizations of Malus crabapple. The quality and quantity of RNA were compared among the three methods. The re- sults showed that RNA extracted by Guanidine Thioeyanate method had good quality and high purity, and the CTAB method had low expense, while Tris-borate method was not fit for RNA extraction from the fruits with polyphenols and polysaccharides organizations.