摘要
应用酶联免疫吸附法(ELISA)测定了番茄酱中链霉素(SMC)和双氢链霉素(DHSMC)的含量,并制备了检测试剂盒。通过戊二醛先后与SMC与牛血清蛋白(BSA)的交联反应制成SMC免疫原(BSA-SMC)。用相同方法,以鸡卵清蛋白(OVA)代替BSA,制得包被原(OVA-SMC)。用免疫原免疫8周龄的雌性BABL/c小鼠制备SMC单克隆抗体(SM-mAb),用OVA-SMC包被酶标板。以百分吸光度(A/A0)为纵坐标,SMC标准溶液的质量浓度为横坐标,得到SMC浓度在0.05～4.05μg.L-1之间呈线性关系。检出限(3s)为1.98μg.kg-1。试验测得回收率在79.4%～92.0%之间,测定值的相对标准偏差(n=6)(批内及批间)均小于10%。DHSMC在此条件也同样反应,故方法测得结果为两者的总量。
Rapid determination of streptomycin （SMC） together with dihydrostreptomycin （DHSMC） in ketchup by the method of enzyme linked immuno sorbent assay （ELISA） was proposed, and the testing reagents kit was prepared. By using pentanedial as cross-linking reagent to react first with SMC and then with BSA and with OVA separately, the SMC immunogen of BSA-SMC, and the coating immunogen of OVA-SMC were prepared. The SMC monoclonal antibody of SMC-mAb was prepared by using the 8 week-aged female BABL/c mousie which was immunized by the SMC immunogen. The OVA-SMC was used to coat the enzyme standard plate. Standard curve was prepared by using the values of absorbance percentage （A/Ao） as ordinate, and the values of mass concentration of SMC standard solution as abscissa, giving a linear relationship in the range of 0. 05 to 4. 05μg·L^-1 of SMC. Detection limit （3s） was found to be 1. 98μg·L^-1. Values of recovery found were in the range of 79. 4%- 92. 0%, and values of RSD＇s （n=6） obtained within batch and among batches were all less than 10%. DHSMC reacted in the same manner as SMC, hence the result of determination was the sum of SMC and DHSMC.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第10期1143-1145,1149,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
新疆出入境检验检疫局科技计划项目(2010xk0048)
