miR-92b-3p在禽流感病毒感染与非感染鸭中的表达差异

Comparison of expression differences of miR-92b-3p in AIV infected and non-infected ducks

采用荧光定量PCR方法检测miR-92b-3p在感染与非感染H5N1亚型禽流感病毒状态下SPF鸭不同组织中的相对表达情况。以筛选到在SPF鸭非感染和感染H5N1亚型禽流感病毒状态下差异microRNA的miR-92b-3p。结果表明:miR-92b-3p在感染与非感染鸭的不同组织中均能检测到表达。在非感染鸭的脾脏中相对表达量最高,感染禽流感病毒后在肺脏中的相对表达量最高;感染禽流感病毒后的SPF鸭与对照组SPF鸭相同组织间的相对比较分析显示,miR-92b-3p在感染病毒鸭的肾脏、气管、肺脏、肝脏和心脏中的表达量均高于SPF鸭的相同组织中的表达量。探明在病毒感染状态下miR-92b-3p对水禽的作用机制,为进一步研究miR-92b-3p预防禽流感病毒感染宿主中的作用提供参考。

The relative expression of miR-92b-3p in different tissues of H5N1 AIV infected and non-infected SPF ducks were examined by quantitative PCR. Different mieroRNA （miR-92b-3p） was obtained from SPF ducks of infected and non-infected H5N1 AIV and based on the analysis of microRNA. The results showed that miR-92b-3p was expressed in all tissues. Furthermore, miR-92b-3p relative expression level was higher in spleen than in other tissues of SPF duck, and at a higher level in lung of H5N1 infected ducks. At the same time, the relative expression level of miR-92b-3p in kidney, trachea, lung, liver and heart of infected SPF ducks was higher than that in non-infected SPF ducks. The research pro- vides the basis for the future studies of miR-92b-3p role in the prevention of avian influenza virus infection in host and the mechanisms of miR-92b-3p in H5N1 infected wild waterfowl.