甲氨蝶呤预防后发性白内障的实验研究

Study of preventing after cararact with methotrexate in vitro

目的 :探讨甲氨蝶呤 (MTX)预防后发性白内障的疗效。方法 :用 YAG激光形成兔眼白内障 ,行白内障囊外摘除术。将 12只兔 (2 4眼 )分为 2组 ,右眼为实验组 ,左眼为对照组 ,实验组手术时在眼用平衡盐溶液 (BSS)中加入 MTX。浓度分别为 2 0 0 ,40 0 ,80 0 mg/ L。对照组则用 BSS灌注。观察术后 3个月时晶体后囊混浊情况 ,并做光镜和电镜检查。结果 :实验组和对照组晶体后囊混浊的差异有显著性 (P<0 .0 5 ) ,用药组仅有 1眼发生轻度后囊混浊 ,眼球各组织结构未见异常。结论 :甲氨蝶呤可预防白内障囊外摘除术后发性白内障形成。

Objective:To investigate the efficacy of methotrexate (MTX) for preventing affer cataract Methods:Cataract model in rabbit eyes were made by Nd YAG laser and estracapsular cataract extraction (ECCE) was under gone later in 12 rabbits(24 eyes) which was then divided into two groups,one of which in right eye was experimental group In this group,the different concentration of MTX was added in the irrigating solutions (BSS) which was 200 mg/L,400 mg/L and 800 mg/L,respectively The left eye in another 12 rabbits was as control,which irrigated only with BSS during ECCE operation The opacity in posterion lens capsule was observed by routine examination,light and electronic microscopy Results:All the eyes in the control group developed opacity in the postenior lens capsule,whereas only one eye in the experimental group developed opacity The difference was significant statistically( P< 0 05) The other tissues in experimental eyes keep normal Conclusions:Addition of MTX in irrigating solution during ECCE may prevent after cataract occurrence